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Rapid detection of gene mutation in isoniazid-resistant M.tuberculosis

Cheng Xiao

Open publisher page 1 citations

Abstract

AIM: To investigate the relationship between the mutation of the katG gene and isoniazid resistance in M. Tuberculosis . METHODS: The specific oligonucleotide primers of the katG gene was designed according to the EMBL:X68081 in genebank. KatG gene mutations were detected by PCR SSCP and DS. H 37 Rv standard isolates were used as control. RESULTS: Compared with the control, no difference was found among the 23 sensitive isolates. Among the 35 resistant isolates, 2 (5.7%) lacked katG gene in those highly INH resistant isolates. The mutation of 23 (65.7%) isolates was detected by the PCR SSCP and that of 24 isolates was detected by DS. The coincidence rate for two the techniques was 95.8% (23/24). CONCLUSION: PCR SSCP is a sensitive and specific method for rapid detection of katG gene mutations in M. tuberculosis and its drug resistance.

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What this paper is about

AIM: To investigate the relationship between the mutation of the katG gene and isoniazid resistance in M. Tuberculosis . METHODS: The specific oligonucleotide primers of the katG gene was designed according to the EMBL:X68081 in genebank. KatG gene mutations were detected by PCR SSCP and DS. H 37 Rv standard isolates were used as control. RESULTS: Compared with the control, no difference was found among the 23 sensitive isolates. Among the 35 resistant isolates, 2 (5.7%) lacked katG gene in those highly INH resistant isolates. The mutation of 23 (65.7%) isolates was detected by the PCR SSCP and that of 24 isolates was detected by DS. The coincidence rate for two the techniques was 95.8% (23/24). CONCLUSION: PCR SSCP is a sensitive and specific method for rapid detection of katG gene mutations in M. tuberculosis and its drug resistance.

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Available abstract

AIM: To investigate the relationship between the mutation of the katG gene and isoniazid resistance in M. Tuberculosis . METHODS: The specific oligonucleotide primers of the katG gene was designed according to the EMBL:X68081 in genebank. KatG gene mutations were detected by PCR SSCP and DS. H 37 Rv standard isolates were used as control. RESULTS: Compared with the control, no difference was found among the 23 sensitive isolates. Among the 35 resistant isolates, 2 (5.7%) lacked katG gene in those highly INH resistant isolates. The mutation of 23 (65.7%) isolates was detected by the PCR SSCP and that of 24 isolates was detected by DS. The coincidence rate for two the techniques was 95.8% (23/24). CONCLUSION: PCR SSCP is a sensitive and specific method for rapid detection of katG gene mutations in M. tuberculosis and its drug resistance.

Key concepts: Isoniazid, Gene, Single-strand conformation polymorphism, Biology, Molecular biology, Gene mutation, Mutation, Tuberculosis

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