The Effect of DES on the Testis Cell of Xiang Pig

The objective of this study was to explore the effects of diethylstilbestrol daily exposure on testis Sertoli cells and germ cells of immaturity Guizhou Xiang-Pig.Twenty male Xiang pig,20-25 days old,were divided into four groups(one control group and three test groups) of 5 each.Test group 1,2 and 3 was received different doses,0.03,0.3 and 3 mg·(kg-1·d-1),of diethylstilbestrol for 9 days by intraperitoneal respectively.24 h after the last injection,the left testis was removed and fixed in 40 g·L-1 paraformaldehyde for 24 h,and then was processed to paraffin wax.Five-micron-thick sections were cut.We used an immunohistochemical method with antibody against GATA-4,a special protein of Sertoli cell,to determine the Sertoli cells,the number of Sertoli and germ cells were counted under microscope.The result showed that DES significantly(P0.05) increased the germ cell number,but significantly(P0.05) decreased Sertoli cell number at 0.3,3(mg·kg-1·d-1) dose,no significance was found at 0.03 mg·(kg-1·d-1) dose of DES(P0.05),compared with that in control group.DES could promote the germ cell proliferation of immature xiang pig testes,while decrease the Sertoli cell proliferation.It was concluded that the DES had different effects on different cell types.