Establishment of Two-dimensional Gel Electrophoresis for Newcastle Disease Virus Infected-cells and Its Preliminary Application

To establish a stable technique of two-dimensional gel electrophoresis(2-DE) for chicken embryo fibroblasts (CEF) infected with Newcastle disease virus(NDV),cell culture,sample preparation,loading capacity,isoelectric focusing,staining and other steps were optimized using NDV-infected CEF as model system. About 800 protein spots on 17 cm IPG strip(pH 4 to 7) stained with blue sliver were analyzed by the software PDQuest8.0.1,and the match rate of protein spots was over 90%. The analysis of multiple 2-DE gels revealed that a total of 36 protein spots differentially expressed during NDV infection,including 16 up-regulated protein spots,15 down-regulated protein spots,3 new emerging protein spots and 2 disappear protein spots. Those differentially expressed protein spots were useful to further investigation of the interaction between NDV and its hosts.