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The lethal effect of As_2O_3 combined with cisplatin on mammary carcinoma cells in vitro

LI Hai-xi

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Abstract

Objective To investigate the lethal effect of As2O3 combined with cisplatin on mammary carcinoma cells in vitro.Methods The single As2O3 or As2O3 combined with cisplatin were used in cultured MCF-7 mammary carcinoma cells.The inhibition rate was measured by MTT.Cellular ultrastructure was observed by electron microscope.Cell apoptosis was examined by flow cytometry.Results 48 hours after the combined application of As2O3 1.0 μg/ml and cisplatin 1.0 μg/ml in MCF-7 cells,the inhibition rate was 27.01%,which was significantly higher than that of the single application of As2O3 1.0 μg/ml(12.12%) or cisplatin 1.0 μg/ml(10.93%).The changes of celluar ultrastructure and flow cytometry suggested that the apoptosis inductive effect of the combined application was much more remarkable than that of the single application(P0.01).Conclusion Combined application of As2O3 and cisplatin can kill effectively MCF-7 cells,which is mainly fulfilled by inducing cell apoptosis.

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Objective To investigate the lethal effect of As2O3 combined with cisplatin on mammary carcinoma cells in vitro.Methods The single As2O3 or As2O3 combined with cisplatin were used in cultured MCF-7 mammary carcinoma cells.The inhibition rate was measured by MTT.Cellular ultrastructure was observed by electron microscope.Cell apoptosis was examined by flow cytometry.Results 48 hours after the combined application of As2O3 1.0 μg/ml and cisplatin 1.0 μg/ml in MCF-7 cells,the inhibition rate was 27.01%,which was significantly higher than that of the single application of As2O3 1.0 μg/ml(12.12%) or cisplatin 1.0 μg/ml(10.93%).The changes of celluar ultrastructure and flow cytometry suggested that the apoptosis inductive effect of the combined application was much more remarkable than that of the single application(P0.01).Conclusion Combined application of As2O3 and cisplatin can kill effectively MCF-7 cells,which is mainly fulfilled by inducing cell apoptosis.

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Available abstract

Objective To investigate the lethal effect of As2O3 combined with cisplatin on mammary carcinoma cells in vitro.Methods The single As2O3 or As2O3 combined with cisplatin were used in cultured MCF-7 mammary carcinoma cells.The inhibition rate was measured by MTT.Cellular ultrastructure was observed by electron microscope.Cell apoptosis was examined by flow cytometry.Results 48 hours after the combined application of As2O3 1.0 μg/ml and cisplatin 1.0 μg/ml in MCF-7 cells,the inhibition rate was 27.01%,which was significantly higher than that of the single application of As2O3 1.0 μg/ml(12.12%) or cisplatin 1.0 μg/ml(10.93%).The changes of celluar ultrastructure and flow cytometry suggested that the apoptosis inductive effect of the combined application was much more remarkable than that of the single application(P0.01).Conclusion Combined application of As2O3 and cisplatin can kill effectively MCF-7 cells,which is mainly fulfilled by inducing cell apoptosis.

Key concepts: Cisplatin, Flow cytometry, Apoptosis, In vitro, Medicine, Ultrastructure, MTT assay, Mammary carcinoma

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