THE DYNAMIC DOSE RESPONSE OF RAT SERUM ANTIOXIDANT SYSTEM TO ALCOHOL AND THE PREVENTIVE EFFECT OF GINKGETIN

Objective: To study the dynamic response of rat serum antioxidant system to ethanol with different doses and the protective role of ginkgetin on redox imbalance . Method: Male SD rats were divided into 7 groups according to their body weights, namely normal control, 3 ethanol groups at low, medium and high dose (0.8, 1.6, or 2.4 g/kg, respectively), low and high interventions (48 or 96 mg/kg ginkgetin + 2.4 g/kg ethanol) and ginkgetin control (96 mg/kg). Serum was analyzed for the contents of glutathione (GSH) and malondialdehyde (MDA), as well as the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) on D 30, 60 and 90. Results: The activities of serum SOD, CAT and GSH-Px increased gradually in rats fed by low dose of ethanol. The activities of SOD and CAT were elevated on D 30, but GSH level decreased and MDA content increased gradually on D 60 and D90 when fed by medium ethanol. The inactive extents of serum antioxidant enzymes, including the degree of GSH depletion and MDA accumulation, were further increased in the presence of high ethanol. Ginkgetintreatment, especially at high dose, effectively inhibited the inactivation of antioxidant enzymes, depletion of GSH, and elevation of MDA level. Conclusion: Low ethanol activates the antioxidant defense system, whereas high ethanol results in evident redox disorder. Ginkgetin exerts favorable regulative and protective effect on the imbalance induced by high ethanol.