Study on extraction process of crude Scutellaria barbata polysaccharide and determine component of monosaccharide

Objective:to optimize the extraction process of crude Scutellaria barbata polysaccharide and to determine the component of monosaccharide.Methods:single factor test and orthogonal experiment design methods were applied to analyze the influence of different factors on the extraction of the crude polysaccharides,adopt gas chromatogram(GC)to determine the component of monosaccharide as well.Results:the optimal temperature was 60℃,solid-liquid ratio was 1∶20,extraction time was 2h and extraction times were 4,the rate of polysaccharides extraction was 2.823%,in while the GC results indicated that crude polysaccharides were composed of seven kinds of monosaccharide.Conclusion:the rate of polysaccharides extraction was increased with optimal process.The optimal process could be stabilization and credibility.The component of crude polysaccharides were identified:viz.asarabinose,rhamnose,ribose,mannose,fructose,galactose and glucose.