Observation of GAGs inhibiting the osteoclastogenesis of RAW264.7 in presence of RANKL

Objective To investigate the effects of glycosaminoglycan(GAGs) on the osteoclastogenesis of macrophage cell line RAW264.7 in presence of receptor activator of nuclear factor-kappa B ligand(RANKL).Methods RAW264.7 was induced by RANKL alone in control group.Different concentration gradients of heparin,hyaluronic acid(HA)and chondroitin sulfate C(CS) combined with RANKL were used in heparin,HA and CS group.Tartrate resistant acid phosphatase(TRAP)staining was used to evaluate the numbers of osteoclast-like cells in 3,5,7,8 days.The quantities of TRAP5b in the medium were detected by ELISA.Results The osteoclast-like cell numbers in the experimental groups decreased compared to the control groups in 3,5,7,8 days(P0.05).The osteoclast-like cell numbers decreased with the increasing of concentration gradients of heparin(P0.05).TRAP5b quantities in medium were the same to the count results in day 8.Conclusion GAGs can inhibit osteoclastogenesis of RAW264.7 cell line in presence of RANKL.The inhibition of heparin is in dose-dependent manner.