2010•Science and Technology of Food IndustryRequires access

Study on the extraction of papain with aqueous two-phase system

Wang Hai-bo

Abstract

The extraction of papain in polyethylene glycol(PEG)/(NH4)2SO4 aqueous two-phase system was investigated.The effects of different molecular weight on the papain activity,and concentration of PEG or(NH4)2SO4,pH and temperature on papain partition coefficient K were studied.The results showed that the optimal aqueous two-phase system for papain extraction was 27% PEG600,21%(NH4)2SO4 at pH7.0,and 60℃.Under above conditions,the partition coefficient K of papain reached 9.28.

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Key concepts: Papain, Partition coefficient, Polyethylene glycol, PEG ratio, Extraction (chemistry), Chemistry, Aqueous solution, Chromatography