Surfactant Coated Lipase as A Catalyst for Esterification Reactions in Organic Solvents

The lipase from Candida rugosa was coated with synthetic glutamic acid dialkyl ester ribitol and used for catalyzing esterification reactions in organic solvents. The esterification of lauric acid and lauryl alcohol was selected as a model reaction. It was found that the optimal pH of buffer for preparation of the surfactant-coated lipase was 6.8 and the best coating agent was glutamic acid didedocyl ester ribitol. The best reaction temperature for the esterification was 30℃, the most suitable organic solvent is isooctane, and the substrate conversion reached 94% within 10h.