APOPTOSIS OF EBV POSITIVE GASTRIC EPITHELIUM CELLS INDUCED BY siRNA TARGETED LMP1 GENE IS ASSOCIATED WITH INHIBITION OF Bcl-2 GENE EXPRESSION

Objective To explore the relationship between specific silencing effect of siRNA that targets latent membrane protein 1(LMP1) coding gene on the proliferation and apoptosis of EBV positive gastric epithelium cells. Methods The chemically synthetic siRNA targeting LMP1 was transfected into target cells,then RT-PCR,Hochest 33258 staining and flow cytometry were used to detect apoptosis and cell cycle of target cells,respectively. Results RT-PCR showed that siRNAs markedly inhibited the expression of LMP1 in target cells,and the effect of siRNA649 was most obvious.Apoptosis was observed in target cells transfected by siRNA649 with Hochest 33258 staining.A flow cytometry analysis showed no obvious discrepancy after 24,72 and 120 h of transfection of siRNA649.RT-PCR showed that siRNA649 inhibited the expression of Bcl-2 in target cells. ConclusionChemically synthetic siRNA targeting LMP1 gene could effectively silence the expression of LMP1.Then it may though suppressing the expression of Bcl-2 lead target cells apoptosis.This cell line can be used as a good target cell to explore LMP1 bioactivity and its effect in oncogenesis of EBV correlated tumors.