2007•Biotechnology(Faisalabad)Requires access
Cloning and Analysis of HCV Core Protein Gene in Pichia pastoris
Zhiyu Wang
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Abstract
Objective: To clone the HCV core protein gene and its upstream DNA for the expression of the gene.Methods: The core protein gene and its upstream DNA were got from HCV total RNA by reverse transcription and PCR.They were linked into pMD18-T vector and were cut down by restriction endonuclease.Then they were inserted into expression vector pPIC9K of Pichia pastoris,and the recombinant plasmids were obtained.After they were identified by sequence,the target gene was cloned and identified.Results: After Pichia pastoris was transformed by the recombinant plasmid,the cloned target gene was confirmed by PCR analysis.Conclusion: The HCV core protein gene was cloned successfully by using pPIC9K as the vector and using Pichia pastoris as host bateria.