Resveratrol on oxidative stress injury of intestinal epithelial cell in obstructive jaundice rats

Objective To investigate the effects of resveratrol(Res)on oxidative stress injury of intestinal epithelial cell in obstructive jaundice(OJ)rats.Methods Sham group was negative control group and solvent group as a positive control group,after two weeks′experiment,the tissue samples were obtained.Healthy male Wistar rats were randomly divided into five groups:control group,model group,solvent group,Res 10 mg group and Res 20 mg group,each group contained 12 rats.OJ was successfully established in model group,solvent group,Res 10 mg group and Res 20 mg group after bile duct ligation.For control group,the choledochal duct was not ligated.Endpoint chromogenic assay with tachypleus amebocyte lysate was used to detect plasma endotoxin levels. HE stain method and transmission electron microscopy were used for morphological changes of intestinal mucosa.Colorimetric detection was made for intestinal tissue malondialdehyde(MDA)and superoxide dismutase(SOD)activity.The apoptosis of mucosal cells was detected by TUNEL methods.Results Two weeks after the experimental treatment,the plasma endotoxin concentration of model group was higher than that of sham operation group(P0.05).The histological examination showed the glands of rats in model group arranged disorderly,villous blunting,shortening,accompanied by many lymphocytes and inflammatory cell infiltration.Transmission electron microscopy showed mucosal microvilli was disarranged and distorted,cell gap widened,fuzzy connection.Compared with sham operation group,the intestinal lipid peroxidation of model group increased;the MDA content in intestinal mucosa of model group was significantly higher than that of sham operation group,but SOD of model group markedly reduced(P0.05).TUNEL analysis showed the intestinal epithelial cell apoptosis index of model group was significantly higher than that of sham operation group.The above pathological changes were corrected after being treated with Res in different concentrations,the content of MDA in intestinal mucosa significantly decreased compared with model group and solvent group(P0.05),but SOD increased(P0.05);The apoptotic index of mucosal cells in Res 20 mg treated group was significantly lower than that in Res 10 mg treated group(P0.05).Conclusion The intestinal epithelial cell in OJ rats results in high oxidative stress injury,Res can protect the intestinal mucosal barrier function in OJ rats through significantly ameliorating intestinal endotoxemia,improving the antioxidant function of intestinal mucosa,inhibiting the apoptosis of intestinal epithelial cells.