Expression of P504S,p63,and CK34bE12 in prostatic carcinoma

Objective:To investigate the significance of expression of P504S,p63 and CK34βE12 in diagnosis and differential diagnosis of prostatic carcinoma.Methods:Immunohistochemical staining was used for detection of expression of P504S,p63, and basal cell-specific anti-cytokeratin antibody(CK34βE12)in various prostatic lesions.Results:P504S was expressed in 92 ca ses out of 102 patients with prostatic carcinoma(90.2%).The positive rate of P504S expression was 8.2%(8/97)in benign prostatic lesions and 39.5%(15/38)in high grade intraepithelial neoplasias(HGPIN).In the removed benign prostatic speci- mens,the occurrence of lack of expression in more than two benign glands of p63 was significantly lower than that of CK34βE12 (28.8% vs 53%,P＜0.05).Conclusion:P504S is a sensitive biomarker of prostatic carcinoma;p63 is more sensitive than CK34βE12 in staining benign prostatic basal cells.Combined detection of P504S/basal cell biomarker may be of greater benefit in clinical diagnosis of prostatic carcinoma.