Genetic diversity of natural Prunus mira populations detected by SRAP

Sequence-related amplified polymorphism(SRAP) markers were used to assess the genetic diversity and population structure of four natural Prunus mira populations from Tibet.A total of 376 bands were amplified from four natural populations by 20 primers,and 338 SRAP loci were found to be polymorphic.A relatively high level of intraspecific genetic diversity was revealed: PPB(percentage of polymorphic loci)=89.89%,H(Nei's gene diversity)=0.194 3,I(Shannon's information index)=0.295 5 at the population level,and PPB=89.89%,H=0.246 0,I=0.383 9 at the species level.A low degree of genetic differentiation occurred among P.mira populations as indicated by a Gst(Nei's Gst analysis) of 0.170 9 but the gene flow between populations was high(1.213 0).UPGMA(unweighted pair group method with arithmetic mean) cluster analysis showed that the 70 individuals were not clustered into four clusters corresponding to the four populations.There was relatively high genetic diversity,low genetic differentiation and a high degree of gene flow between P.mira populations.