The Relations between Genetic Polymorphism and Mastitis Resistance of Holstein Cattle HSP 70-1 Gene

With PCR-RFLP and PCR-SSCP to detect the polymorphism of HSP70-1 gene in Holstein cattle.And to analyze the polymorphism and somatic cell score(SCS) relevance.The results showed that HSP70-1 gene amplification 1 623 bp points of the mutations produced G-A,1 623 bp produce the G-A-C mutation.Two Locus are silent mutation,did not change the amino acid sequence.HSP70-1 genes performed polymorphism after digestion of the EcoR Ⅱ,Bgl Ⅰ,Fok Ⅰaccording to χ2 test,China Holstein in the site does not reach the Hardy-Weinberg equilibrium.At the same time,the correlation analysis of different genotypes and SCS showed that there is no significnt correlation between 2 409 locus genotype and SCS,but there is a significant corrclation between 1 623 locus and SCS.The index of CCSCS was significantly lower than that of AG,GG-(P0.05),CC genotype can be as the favorable genotype of mastitis resistance,HSP70-1 gene cow can be as candidate genes of cow mastitis,which will be applied to molecular marker-assisted selection and breeding,which lays the theoretical foundation of breeding cattle.