Effects of XiaoBanXia Decoction on Movement of Isolated Ileum in Guinea Pig

Objective: To observe the effect of Xiaobanxia decoction on the movement of isolated ileum in guinea pig and analyze the mechanism of anti-emetic effect of Xiaobanxia decoction(XBXD).Method: The effects of XBXD(0,0.1,0.25,0.5,1,2.5,5,10,20,40 g·L-1) on ileum spontaneous contraction tension,amplitude and frequency were investigated in organ bath.Emesis-relating agonists acetylcholine(Ach),histamine(His),5-hydroxytryptamine(5-HT),dopamine(DA) were used as tool drugs,the inhibitory effect of XBXD(0,2,5,10 g·L-1) on contraction tension,amplitude and frequency of isolated ileum in guinea pig were observed.Result: XBXD could dose-dependently inhibit ileum spontaneous contractions,reducing the average tension,amplitude,frequency(P 0.05 or P 0.01 vs control).Ach,His and 5-HT could stimulate ileum contraction,enhance the tension,amplitude and frequency.All the three doses(10,5,2 g·L-1) of XBXD could suppress the ileum contraction stimulated by above three tool drugs,significantly reduced the contraction tension,amplitude and frequency(P 0.05 or P 0.01 vs before XBXD or XBXD 0 g·L-1).DA could inhibit the spontaneous contraction,the middle and small dose(5,2 g·L-1) of XBXD could antagonize the inhibitory effect of DA,and enhance the contraction tension,amplitude and frequency(P 0.05 or P 0.01 vs before XBXD or XBXD 0 g·L-1).Conclusion: XBXD can inhibit spontaneous contractile activity of ileum in guinea pig,the mechanism may be related to blocking M,H1,5-HT and D2receptors or directly suppressing ileum smooth muscle.The mechanism of anti-emetic effect of XBXD needs further study.