Research on the detection methods of Cryptosporidium for human and animal feces
Cui Xu-ch
Abstract
Cui Xu-ch
Abstract
Objective To know the advantages and disadvantages of RIDAQuick Cryptosporidium method for the Cryptosporidium detection among feces of human and live stocks,and find out the consistency to auramine-phenol modified acid-fast staining method.Methods 200 and 72 feces of human and live stocks were separately collected. All feces were detected by RIDAQuick Cryptosporidium method and auramine-phenol modified acid-fast staining. Chi-square test was used to analyze the results of the two detection methods by SPSS. Results The positive rates of people and live stocks using RIDAQuick Cryptosporidium were 5% and13. 9%,respectively. And the positive rates of human and live stocks using auramine-phenol modified acid-fast staining method were4. 5% and 25%,respectively. Two methods for human feces detection were of no significant difference( P 0. 05),however,there were significant differences between two methods for the detection of livestock feces( P 0. 05). Conclusions RIDAQuick Cryptosporidium method had good results for Cryptosporidium detection in a large sample of human feces.
A significance statement is not available in the OpenAlex record.
A contribution statement is not available in the OpenAlex record.
Method details are not available in the OpenAlex metadata.
Findings are not separately available in the OpenAlex metadata.
Limitations are not available in the OpenAlex metadata.
Application details are not available in the OpenAlex metadata.
Objective To know the advantages and disadvantages of RIDAQuick Cryptosporidium method for the Cryptosporidium detection among feces of human and live stocks,and find out the consistency to auramine-phenol modified acid-fast staining method.Methods 200 and 72 feces of human and live stocks were separately collected. All feces were detected by RIDAQuick Cryptosporidium method and auramine-phenol modified acid-fast staining. Chi-square test was used to analyze the results of the two detection methods by SPSS. Results The positive rates of people and live stocks using RIDAQuick Cryptosporidium were 5% and13. 9%,respectively. And the positive rates of human and live stocks using auramine-phenol modified acid-fast staining method were4. 5% and 25%,respectively. Two methods for human feces detection were of no significant difference( P 0. 05),however,there were significant differences between two methods for the detection of livestock feces( P 0. 05). Conclusions RIDAQuick Cryptosporidium method had good results for Cryptosporidium detection in a large sample of human feces.
Key concepts: Cryptosporidium, Feces, Human feces, Biology, Staining, Veterinary medicine, Significant difference, Microbiology