2015Journal of Public Health and Preventive MedicineRequires access

Research on the detection methods of Cryptosporidium for human and animal feces

Cui Xu-ch

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Abstract

Objective To know the advantages and disadvantages of RIDAQuick Cryptosporidium method for the Cryptosporidium detection among feces of human and live stocks,and find out the consistency to auramine-phenol modified acid-fast staining method.Methods 200 and 72 feces of human and live stocks were separately collected. All feces were detected by RIDAQuick Cryptosporidium method and auramine-phenol modified acid-fast staining. Chi-square test was used to analyze the results of the two detection methods by SPSS. Results The positive rates of people and live stocks using RIDAQuick Cryptosporidium were 5% and13. 9%,respectively. And the positive rates of human and live stocks using auramine-phenol modified acid-fast staining method were4. 5% and 25%,respectively. Two methods for human feces detection were of no significant difference( P 0. 05),however,there were significant differences between two methods for the detection of livestock feces( P 0. 05). Conclusions RIDAQuick Cryptosporidium method had good results for Cryptosporidium detection in a large sample of human feces.

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Objective To know the advantages and disadvantages of RIDAQuick Cryptosporidium method for the Cryptosporidium detection among feces of human and live stocks,and find out the consistency to auramine-phenol modified acid-fast staining method.Methods 200 and 72 feces of human and live stocks were separately collected. All feces were detected by RIDAQuick Cryptosporidium method and auramine-phenol modified acid-fast staining. Chi-square test was used to analyze the results of the two detection methods by SPSS. Results The positive rates of people and live stocks using RIDAQuick Cryptosporidium were 5% and13. 9%,respectively. And the positive rates of human and live stocks using auramine-phenol modified acid-fast staining method were4. 5% and 25%,respectively. Two methods for human feces detection were of no significant difference( P 0. 05),however,there were significant differences between two methods for the detection of livestock feces( P 0. 05). Conclusions RIDAQuick Cryptosporidium method had good results for Cryptosporidium detection in a large sample of human feces.

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Available abstract

Objective To know the advantages and disadvantages of RIDAQuick Cryptosporidium method for the Cryptosporidium detection among feces of human and live stocks,and find out the consistency to auramine-phenol modified acid-fast staining method.Methods 200 and 72 feces of human and live stocks were separately collected. All feces were detected by RIDAQuick Cryptosporidium method and auramine-phenol modified acid-fast staining. Chi-square test was used to analyze the results of the two detection methods by SPSS. Results The positive rates of people and live stocks using RIDAQuick Cryptosporidium were 5% and13. 9%,respectively. And the positive rates of human and live stocks using auramine-phenol modified acid-fast staining method were4. 5% and 25%,respectively. Two methods for human feces detection were of no significant difference( P 0. 05),however,there were significant differences between two methods for the detection of livestock feces( P 0. 05). Conclusions RIDAQuick Cryptosporidium method had good results for Cryptosporidium detection in a large sample of human feces.

Key concepts: Cryptosporidium, Feces, Human feces, Biology, Staining, Veterinary medicine, Significant difference, Microbiology

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