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2013•Zhongguo shiyan fangjixue zazhiRequires access

Study on HPLC Fingerprint of Alkaloids in Croton tiglium

Zhenling Zhang

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Abstract

Objective:To study chromatography fingerprints of the alkaloids in Croton tiglium by HPLC.Method:Waters Symmetry C18 column(4.6 mm×250 mm,5 μm) was used with methanol-0.2% acetic acid of water gradient elution,at flow-rate of 0.8 mL · min-1,detection wavelength at 292 nm;column temperature was at 25 ℃.Result: HPLC was used to establish fingerprint chromatograph of methanol extraction from thirteen batches of C.tiglium,which had been harvested from the same area at the same time.Crontonoside and magnoflorine in the fingerprint were identified.The result showed that peaks were common.Conclusion: The method could be used for the research and the quality control of C.tiglium.

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What this paper is about

Objective:To study chromatography fingerprints of the alkaloids in Croton tiglium by HPLC.Method:Waters Symmetry C18 column(4.6 mm×250 mm,5 μm) was used with methanol-0.2% acetic acid of water gradient elution,at flow-rate of 0.8 mL · min-1,detection wavelength at 292 nm;column temperature was at 25 ℃.Result: HPLC was used to establish fingerprint chromatograph of methanol extraction from thirteen batches of C.tiglium,which had been harvested from the same area at the same time.Crontonoside and magnoflorine in the fingerprint were identified.The result showed that peaks were common.Conclusion: The method could be used for the research and the quality control of C.tiglium.

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Available abstract

Objective:To study chromatography fingerprints of the alkaloids in Croton tiglium by HPLC.Method:Waters Symmetry C18 column(4.6 mm×250 mm,5 μm) was used with methanol-0.2% acetic acid of water gradient elution,at flow-rate of 0.8 mL · min-1,detection wavelength at 292 nm;column temperature was at 25 ℃.Result: HPLC was used to establish fingerprint chromatograph of methanol extraction from thirteen batches of C.tiglium,which had been harvested from the same area at the same time.Crontonoside and magnoflorine in the fingerprint were identified.The result showed that peaks were common.Conclusion: The method could be used for the research and the quality control of C.tiglium.

Key concepts: Chromatography, Chemistry, Gradient elution, High-performance liquid chromatography, Fingerprint (computing), Extraction (chemistry), Croton, Column chromatography

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Study on HPLC Fingerprint of Alkaloids in Croton tiglium — Research Paper | ScholarLens