Preliminary Study about Reconstruction of the Structure and Function of Decellular Matrix Kidney

Objective: This experiment is only a preliminary study to explore the optimal dose of decellular agents and perfusion programs by a semi-automatic renal perfusion to elute parts of cells and matrix of renal.Methods: Different decellular agents in 10-15 ml/min velocity and temperature conditions were perfused into healthy rabbit kidneys through renal artery by semi-automatic pump perfusion system.Changes in the course of renal perfusion should be observed.Kidneys were dissected and underwent hematoxylin-eosin stain to observe the general histological examination,focusing on glomerular and tubular cell structure changes.Results: The best decellular agent and perfusion scheme is 1 % collagenase Ⅳ at 37 ℃ for 20min,the enzyme can elute renal parenchyma cells within a relatively little time without side injury to renal matrix,and it can reduce the warm ischemia time of renal,which contributes to kidney regeneration.The second best decellular agent and perfusion scheme is 0.5 % trypsin at 25 ℃ for 2h,which can elute more glomerular cells,leave only the matrix part and have little effect on renal tubular,but this perfusion scheme with a longer time,has reached the resistance limit of kidney in vitro to ischemia.Thus,it is not recommended.The most powerful decellular agent is SDS,but the renal caused irreversible damage,especially the damage of basement membrane is not conducive to regeneration of renal.Other perfusion schemes are not ideal.Conclusion: By selecting the appropriate decellular agent,with a suitable temperature,concentration,perfusion pressure and other conditions,parts of renal cells and matrix can be eluted,which will provide an experimental basis for the reconstruction of the structure and function of nonfunctioning kidney.