Knockdown of survivin expression using siRNA inhibits the growth of PC-3M cells

AIM:To study the inhibitory effects of survivin siRNAs on the growth of PC-3M cells.METHODS:Two pairs of DNA template coding siRNA against survivin were synthesized to construct two recombinant plasmids,pSi-sur1 and pSi-sur2.The two recombinants and the two controls,lipofectin and vacant plasmid were transfected into PC-3M cells.The expressions of survivin mRNA and protein were detected respectively by RT-PCR and Western blotting.Proliferation abilities were measured by MTT,and the cell cycle and apoptosis were assayed by FCM.RESULTS:After 72 h of transfection,the level of cell survivin mRNA in the two siRNA groups was 48%±6%(n=3)and 30%±5%(n=3)of that in lipofectin group,and expression of survivin protein were 38%±4%(n=3)and 36%±4%(n=3)respectively of that in lipofectin control.The proliferation rate of cells in pSi-sur1 and pSi-sur2 groups was also inhibited according to MTT,about 44.20%±2.08%(n=3)and 39.20%±1.93%(n=3)of that in lipofectin group.Cell numbers of G1 phase in two siRNA groups were significantly higher than that in two controls,while cells of G2 phase and S phase were much lower.Cell apoptosis was found in both siRNA groups.CONCLUSION:The two survivin siRNA significantly inhibit the expression of survivin in mRNA and protein levels,arrest the cell cycle in G1 phase,and suppress the growth of PC-3M cells and induce apoptosis in vitro.