2013•Beijing Linye Daxue xuebaoRequires access
Somatic embryogenesis and plant regeneration from immature zygotic embryos of Prunus mume ‘Xue Mei'
Yang Ji
Open publisher page 0 citations
Abstract
Using immature zygotic embryos of ‘Xue Mei'as explants,the effects of different sampling dates and combinations of 2,4-D and 6-BA on somatic embryogenesis were investigated. The results indicated that the highest induction rate of 58.3% was obtained from immature embryos of‘Xue Mei'collected on the 59th day after flowering and incubated for 60 d on the 1/2 MS + 1.0 mg/L 2,4-D + 0.5 mg/L 6-BA in the dark. The maximum average number of somatic embryos could reach 3.6 on the 1/2 MS + 1.0 mg/L2,4-D + 0.2 mg/L 6-BA. Secondary somatic embryos could be produced when primary somatic embryos were cultured on proliferation medium 1/2 MS + 0.1 mg/L 6-BA + 0.05 mg/L NAA,and plantlets generated from somatic embryos on germination medium 1/2 MS + 1.0 mg/L 6-BA + 0.1 mg/L NAA + 0.5mg/L GA3.