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2000•Unpublished venueRequires access

Construction of phage antibody library and screening of human anti-ATP monoclonal antibody

Xiao‐Jun Tian

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Abstract

Objective To isolate human anti ATP Fab fragment from phage antibody library. Methods Human immunoglobulin heavy chain Fd genes and κ chain genes were amplified by RT PCR and phage antibody library was constructed. Human anti AFP Fab was obtained and its binding activity to AFP was tested by ELISA. Results The objective fragment was obtained and its specificity was identified by ELISA. Conclusion The successful isolation of human anti ATP Fab proves the feasibility of phage display system in human McAb preparation and founds a basis for the further characterization and clinical application of the Fab.

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Objective To isolate human anti ATP Fab fragment from phage antibody library. Methods Human immunoglobulin heavy chain Fd genes and κ chain genes were amplified by RT PCR and phage antibody library was constructed. Human anti AFP Fab was obtained and its binding activity to AFP was tested by ELISA. Results The objective fragment was obtained and its specificity was identified by ELISA. Conclusion The successful isolation of human anti ATP Fab proves the feasibility of phage display system in human McAb preparation and founds a basis for the further characterization and clinical application of the Fab.

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Available abstract

Objective To isolate human anti ATP Fab fragment from phage antibody library. Methods Human immunoglobulin heavy chain Fd genes and κ chain genes were amplified by RT PCR and phage antibody library was constructed. Human anti AFP Fab was obtained and its binding activity to AFP was tested by ELISA. Results The objective fragment was obtained and its specificity was identified by ELISA. Conclusion The successful isolation of human anti ATP Fab proves the feasibility of phage display system in human McAb preparation and founds a basis for the further characterization and clinical application of the Fab.

Key concepts: Phage display, Antibody, Monoclonal antibody, Immunoglobulin Fab Fragments, Immunoglobulin light chain, Molecular biology, Peptide library, Virology

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