Toxicity and control efficiency of fungicides against Botrytis cinerea

【Objective】The toxicity of Bacillus amyloliquefaciens wettable powder(WP)on Botrytis cinerea and the control effect on tomato gray mold were studied to improve the application of Bacillus amyloliquefaciens.【Method】 The inhibitory effects of five fungicides including 1 × 109 CFU/g B.amyloliquefaciens WP,1×109 CFU/g B.subtilis WP,40% carbendazim WP,50% iprodione WP and40% pyrimethanil WP against tomato gray mold pathogen were determined using hyphae growth rate method and spore germination method,and their control effects were determined in pot and field experiments.【Result】The 50%effective concentrations(EC50)of B.amyloliquefaciens,B.subtilis,40%carbendazim,50%iprodione and 40% pyrimethanil against the growth of the hyphae were 946.28,757.19,75.36,8.95 and 5.53μg/mL,respectively,and their EC50 against the germination of conidia were 1 665.56,1 416.16,241.90,37.32 and 21.35μg/mL,respectively.The control effects of five fungicides(diluted 750 times solution)were 68.47%,70.14%,74.18%,74.13% and 80.63% in pot experiment and 63.37%,64.83%,69.56%,73.49% and 75.55%in field experiment.【Conclusion】The toxicity and control effect B.amyloliquefaciens WP in field experiment of were equivalent to those of B.subtilis WP.Therefore,B.amyloliquefaciens WP can be used as assistant biofungicides to control tomato gray mold.