Influence of rapamycin on the biological behaviour of osteosarcoma cells and its molecular mechanism in vitro

Objective To observe the influence of rapamycin on the proliferation,apoptosis and invasive ability of osteosarcoma cells and its molecular mechanism in vitro.Methods Human osteosarcoma cells line,MG-63,was cultured in vitro and different concentrations of rapamycin were applied.The cell proliferation was detected by MTT assay.The apoptosis rate was measured by flow cytometry.The invasive ability was observed by transwell.Western-blot assay was used to detect the expression of bcl-2 and VEGF protein after treated by rapamycin.Results The proliferation and invasive ability decreased and the apoptosis rate increased after treated by rapamycin.The protein expressions of bcl-2 and VEGF were significantly decreased after the use of rapamycin.Conclusion Rapamycin could induce apoptosis,inhibit the proliferation and invasive ability of osteosarcoma by the down-regulation expression of bcl-2 and VEGF.