Effect of nimesulide on inducing apoptosis of gastric cancer cell

Objective To investigate the effect of apoptosis of gastric cancer cell line SGC7901 treated with selective cyclooxgenase 2(COX-2) inhibitor nimesulide and the probable mechanism of cell signal transduction.Methods SGC7901 cells were treated with nimesulide.Flow cytometry was used to detect cell cycle and apoptosis.The protein expression related to STAT3 signaling pathway in SGC-7901 cells was examined by Western blot.Results The percentage of apoptosis increased significantly;Western blot analysis showed that protein expression of phosphorylated STAT3(P-STAT3),bcl-2 in SGC-7901 cells decreased significantly.Conclusion Nimesulide could induce cell apoptosis through down-regulating the protein expression of P-STAT3,bcl-2,which is probably one of its molecular mechanisms.