Effects of adenovirus vector carrying murine IL-12 gene on expression of GATA-3 in lung tissue of mouse model with asthma

Objective To investigate the effects of adenovirus vector carrying murine interleukin-12 gene(AdCMVIL-12) on the expression of GATA-3 in mouse model with asthma.Methods Thirty BALB/c mice were randomly divided into 3 groups: control group(group A),asthma model group(group B) and AdCMVIL-12 experimental group(group C).Asthma models were established in group B and C,saline(50 μL) and 5×108 PFU AdCMVIL-12 were given by intranasal administration at the 20th days.BALF were recovered at the 28th day for cellular classification.Pathological changes of bronchial and lung tissue were observed by HE staining in each groups.The expression of GATA-3 in each group was detected by immunohistochemistry and RTPCR.Results Compared with group A and C,a lot of inflammatory cells were found in group B and the percent of eosinophils was the most,the expression of GATA3 in lung tissue of group B(0.48 ±0.06) was higher than those in group C(0.16±0.07) and group A(0.18±0.04)(P0.01).Conclusion AdCMVIL-12 can significantly inhibit airway and lung tissue inflammation in asthma model,its mechanism may be related to blocking the expression of GATA-3 in asthma model and regulating the balance of Th1/Th2 cytokines.