Study on the biological characteristics of mesenchymal stem cells from human gastric cancer

Objective After isolating and culturing mesenchymal stem cells from human gastric cancer(hGC-MSCs),the biological characters,such as morphological and proliferative characteristics,surface markers,multi directional differentiation potential were analysed,so as to providing scientific data for the research of mesenchymal stem cells from human gastric cancer,(hGC-MSCs).Methods Using enzyme digestion methods to isolate and culture hGC-MSCs from fresh human gastric cancer tissue,hGC-MSCs morphology and proliferative characteristics were observed,growth curve were measured,surface markers were detected by the flow cytometry,and potentiality of multi-directional differentiation about hGC-MSCs was also studied.Results hGC-MSCs can be successfully isolated and cultured in vitro.Cells were observed under an inverted microscope,and appeared spindle-shape and polygon like the fibroblast showing whirlpool-form.The proliferative ability of hGC-MSCs is quite strong and declined with passages.MSCs marker CD29、CD44 and CD90 were high positive,but CD45、CD34 and HLA-DR were lower expressed or negative in hGC-MSCs.hGC-MSCs can differentiate into lipocyte and osteoblast in differentiation medium.Conclusinon There are hGC-MSCs in human gastric cancer,which with the biological characters similar as the characters of MSCs derived from normal adult bone marrow.Study on isolating and culturing hGC-MSCs will be much valuable for gastric cancer research and clinical application.