Clinical efficiency of radiotherapy combined with self-immune cell therapy for cervical carcinoma

Objective: To compare changes of cellular immune function and survival of cervical carcinoma patients receiving radiotherapy combined with self-immune cell therapy with those receiving radiotherapy alone.Methods: Sixty-eight cases of cervical carcinoma were divided into 2 groups: 38 cases in radiotherapy group;30 cases in combination group(radiotherapy combined with self-immune cell therapy).Peripheral blood mononuclear cells(PBMCs) were separated,and then CD3AK cells,cytokine-induced killer cells(CIKs),dendritic cells(DCs),γδT cells and NK cells were induced and cultured.CD3+,CD4+,CD8+,CD19+,CD16+CD56+ and γδT cell numbers and ratios as well as the rate of positive expression of perforin,granzyme B(GraB),and CD107a of PBMCs were evaluated by FCM before and after treatment.The patients were followed by 5 years follow up study.Results: In most patients of the combination group,the quality of life was improved.The scores of Karnofsky in the combination group were significantly higher than those in the radiotherapy group(P0.05).The immune cell numbers in the combination group were significantly higher than those in the radiotherapy group(P0.05).The rates of the positive expression of perforin,GraB and CD107a of PBMCs were higher in the combination group than in the radiotherapy group correspondingly(P0.05).The over-all 1-,2-and 5-year survival rates of Ⅰb-Ⅳ stage patients were 93.3%(28/30),83.3%(25/30) and 76.6%(23/30) in the combination group,and were 86.82%(33/38),68.4%(26/38) and 57.9%(22/38) in the radiotherapy group,respectively(P0.05) with Ⅱb and Ⅲ stage patients showing the most significance.Conclusion: Radiotherapy combined with self-immune cell therapy for cervical carcinoma can enhance the immune function and increase the survival.