Cloning and Expression of α-glucosidase Gene from Thermotoga neapolitana and Study on the Enzymatic Properties

We get the α-glucosidase gene from Thermotoga neapolitana by molecular cloning,then the gene was cloned and expressed in E.coli BL21(DE3).The target gene was amplified by PCR and connected to the expression vector pET-28a.The recombinant plasmid pET-28a-glu was constructed and transferred into E.coli BL21(DE3).Obtained the recombinant protein and extracted crude enzyme.Sequence analysis indicated that the length of the gene was 2166bp.The result of the SDS-PAGE showed that the gene was effectively expressed in E.coli BL21(DE3).The enzyme's optimum reaction temperature was at 70 ℃ and the pH value was about 5.0.