The Research on Bone Marrow Mesenchymal Stem Cells Repairing Rabbit Skin Wound Defect

Objective To study the ability of differentiation of bone marrow mesenchymal stem cells(MSCs) into epidermal cells in the skin wounds and the effects of MSCs on healing rabbit skin wounds and attending skin's regeneration so as to provide a new sourse of seeding cells for clinical skin wound repair. Methods The bone marrow mesenchymal stem cells of rabbits were cultured and amplified in vitro,the MSCs were labeled with BrdU(5-bromodeoxy-uridine).Full skin loss wound was created on both sides of the back of the rabbit whose bone marrow was obtained.The labeled MSCs with collagen as the vector were regrafted back to donor animal left wound(treat group),only collagen in right wound (control group).To observe wound healing,the wound central tissue sample were harvested at 4,5 post-operation weeks and were immunohistochemically stained by BrdU for comparative study. Results There was very significant difference upon contraction rate of wound and healing time of wound between both groups(P0.05).The higher contract rate and the quicker healing speed appeared in treat group.The result from the hematoxylin and eosin staining showed that the epidermis is thicker in treat group than that in control group.A few BrdU positive cells appeared on the hair follicle of the rengenerate skin and the stratum basale and the stratum spinosum of the rengenerate epidermis. Conclusion MSCs have positive effects on healing skin wound,MSCs are able to differentiate into epidermal cells in the skin wounds and take part in the progress of skin's regeneration.