THE PROTECTIVE EFFECT OF HYDROGEN-RICH SALINE ON LIVER INJURY IN RATS WITH OBSTRUCTIVE JAUNDICE

Objective Hydrogen has been reported to selectively reduce the hydroxyl radical and alleviate acute oxidative stress in many models.Meanwhile,hydrogen-rich saline,which is easy and safe to apply,can avoid losing hydrogen into environment and provide higher and more accurate concentration of hydrogen.In this study,we investigated the effects of hydrogen-rich saline on the prevention of liver injury induced by obstructive jaundice in rats.Male Sprague-Dawley rats(n=36)were divided randomly into three experimental groups:sham operated,BDL plus saline treatment(5 ml/kg,i.p.),BDL plus hydrogen-rich saline treatment(5 ml/kg,i.p.).The liver damage was detected microscopically 10 d after BDL.In addition,serum ALT,AST,endotoxin,and HMGB1 levels,tissue MDA content,MPO activity,TNF-α,IL-1β,IL-6,and HMGB1 levels were all increased significantly by BDL.Hydrogen-rich saline reduced these markers and relieved morphological liver injury(P0.01).In conclusion,hydrogen-rich saline attenuates BDL induced liver damage possibly by reduction of oxidative stress and inflammatory reaction.