2013•Zhongguo laonianxue zazhiRequires access
Effects of Qihuangmingmu capsule on retinal cell apoptosis in diabetic mice
Hui Zhang
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Abstract
Objective To investigate the effects of Qihuangmingmu capsule( QHMM) on retinal cell apoptosis in diabetic mice. Methods KK /Upj-Ay mice were randomly divided into model,QHMM( low,middle,high) groups. C57BL /6 mice were selected as control group. Mice were given intragastrically for 3 months. Retina of mice was taken. Apoptotic rate was detected by FCM. Real Time PCR and Western blot were used to assess Bcl-2,Bax,Caspase-3,Fas,FasL mRNA and protein expression in retina. Results Apoptotic rate of retina,the expression of Bax and caspase were lower in QHMM group than those of model mice. The expression of Bcl-2,the ratio of Bcl-2 / Bax were higher in QHMM group than those of model mice. The expression of Fas and FasL were not obviously changed. Conclusions QHMM inhibits cell apoptosis by up-regulating Bcl-2 expression and down-regulating Bax and caspase-3 expression in retina of diabetic mice, then has protective effects on diabetic retinopathy.