Cloning and sequence analysis of the specific mitochondrial DNA fragment related to cytoplasmic male sterility in pepper

【Objective】 Three different types of cytoplasmic male sterile mechanism in pepper were investigated to find out the DNA fragment related to cytoplasmic male sterility.【Method】 Pepper mitochondrial DNA(mt DNA) was extracted from green leaves of cytoplasmic male sterile line and its maintainer line based on high salt-proteinase K method.The specific fragments at around 330 bp were amplified by PCR using special primers designed on the basic of pepper cytoplasmic male sterility associated gene-orf456 in three kinds of cytoplasmic male sterile lines.【Result】 Further sequences analysis showed that these three fragments are identical and termed CMS330 of 99% homology with orf456.【Conclusion】 It reflected that they have the similar male sterile mechanism.