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2014•Trauma and Critical Care MedicineRequires access

Effect of ZnSO_4 Precondition on Myocardium Celluar Cx43 Protein during Ischemia / Reperfusion and Reperfusion Ventricular Arrhythmias in Rats

Hao Ma

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Abstract

Objective To investigate the arrhythmia and the phosphorylation of gap junction protein Connexin43( Cx43) during the ischemia / reperfusion injury in rat heart and effects of ZnSO4( zinc) precondition on it. Methods The male SD rats were randomly divided into 4 groups: sham with saline precondition group( Sham + Sa),sham with ZnSO4precondition group( Sham + Zn),ischemia / reperfusion with saline precondition group( I / R + Sa),ischemia / reperfusion with ZnSO4precondition group( I / R + Zn)( n = 6 in each). The incidence of ventricular arrhythmias and arrhythmia score were observed and the phosphorylation level of Cx43 was evaluated by Western-Blot in each group. Results Compared with the Sham + Sa group and Sham + Zn group,ventricular arrhythmia score in I / R + sa group was obviously increased( P 0. 01),and the phosphorylation level of Cx43 in ventricular myocardial cells was also significantly increased( P 0. 01). Compared with the I / R + Sa group,ventricular arrhythmia score in I / R + Zn group obviously decreased( P 0. 01),and the phosphorylation level of Cx43 in ventricular myocardial cells was also significantly decreased( P 0. 01). Conclusion ZnSO4precondition can effectively reduce the incidence of reperfusion ventricular arrhythmias which may be associated with the modulation of Cx43 phosphorylation during ischemia / reperfusion in rats.

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Objective To investigate the arrhythmia and the phosphorylation of gap junction protein Connexin43( Cx43) during the ischemia / reperfusion injury in rat heart and effects of ZnSO4( zinc) precondition on it. Methods The male SD rats were randomly divided into 4 groups: sham with saline precondition group( Sham + Sa),sham with ZnSO4precondition group( Sham + Zn),ischemia / reperfusion with saline precondition group( I / R + Sa),ischemia / reperfusion with ZnSO4precondition group( I / R + Zn)( n = 6 in each). The incidence of ventricular arrhythmias and arrhythmia score were observed and the phosphorylation level of Cx43 was evaluated by Western-Blot in each group. Results Compared with the Sham + Sa group and Sham + Zn group,ventricular arrhythmia score in I / R + sa group was obviously increased( P 0. 01),and the phosphorylation level of Cx43 in ventricular myocardial cells was also significantly increased( P 0. 01). Compared with the I / R + Sa group,ventricular arrhythmia score in I / R + Zn group obviously decreased( P 0. 01),and the phosphorylation level of Cx43 in ventricular myocardial cells was also significantly decreased( P 0. 01). Conclusion ZnSO4precondition can effectively reduce the incidence of reperfusion ventricular arrhythmias which may be associated with the modulation of Cx43 phosphorylation during ischemia / reperfusion in rats.

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Available abstract

Objective To investigate the arrhythmia and the phosphorylation of gap junction protein Connexin43( Cx43) during the ischemia / reperfusion injury in rat heart and effects of ZnSO4( zinc) precondition on it. Methods The male SD rats were randomly divided into 4 groups: sham with saline precondition group( Sham + Sa),sham with ZnSO4precondition group( Sham + Zn),ischemia / reperfusion with saline precondition group( I / R + Sa),ischemia / reperfusion with ZnSO4precondition group( I / R + Zn)( n = 6 in each). The incidence of ventricular arrhythmias and arrhythmia score were observed and the phosphorylation level of Cx43 was evaluated by Western-Blot in each group. Results Compared with the Sham + Sa group and Sham + Zn group,ventricular arrhythmia score in I / R + sa group was obviously increased( P 0. 01),and the phosphorylation level of Cx43 in ventricular myocardial cells was also significantly increased( P 0. 01). Compared with the I / R + Sa group,ventricular arrhythmia score in I / R + Zn group obviously decreased( P 0. 01),and the phosphorylation level of Cx43 in ventricular myocardial cells was also significantly decreased( P 0. 01). Conclusion ZnSO4precondition can effectively reduce the incidence of reperfusion ventricular arrhythmias which may be associated with the modulation of Cx43 phosphorylation during ischemia / reperfusion in rats.

Key concepts: Ischemia, Medicine, Internal medicine, Cardiology, Saline, Phosphorylation, Reperfusion injury, Western blot

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Effect of ZnSO_4 Precondition on Myocardium Celluar Cx43 Protein during Ischemia / Reperfusion and Reperfusion Ventricular Arrhythmias in Rats — Research Paper | ScholarLens