Anatomy Structures of Leaves and Stems in the Dwarfing Mutant Soybean

Dwarf,a excellent agronomic index,is thought to play important roles in improving the growing density and enhancing the efficiency of photosynthesis.The soybean dwarf mutant Dongze 11 was obtained from Dongnong 42 treated with NaN3.Anatomic structures of leaves and stem were observed by paraffin-slice-up in mutant soybean and wild type.The results indicated that shorter transverse length of parenchyma cell (width),longer vertical length and smaller parenchyma cell number per unit area (no significant difference) were detected in dwarf mutant.Morphological measurements revealed that blade-thickness,palisade tissue-thickness,spongy-thickness and proportion of spongy-thickness and palisade tissue-thickness were all smaller in the dawarf mutant plants,and those characters was notably different in shorter dawarf plants.The numbers of main vascular bundle,vessel number,vessel diameter,area of transporting water and area of phloem in main vein of leaves were lower in the dawarf mutant soybean.In the present study,the dwarfing seedlings were screened,based on tissue propotion of spongy thickness and palisade thickness.