Cellular immune responses of BALB/c mice induced by GPV-VP3 DNA vaccine immunized intramuscular injection with different dose

BALB/c mice were immunized with 50,100,200 μg of pcDNA-GPV-VP3 DNA vaccine respectively by the route of intramuscular injection,pcDNA3.1(+)and physiological saline as controls.Collected the peripheral blood samples at 7,14,21,28,35,63 and 105 d after immunizaton.T lymphocyte proliferation test(MTT)was used to detect the proliferation of T-lymphocytes cell,Fluorescence activated cell sorter(FACS)was used to detect the number of CD4+,CD8+ cells in peripheral blood lymphocytes.Results:pcDNA-GPV-VP3 DNA vaccine induced good cellular immune responses.①ConA response of T-lymphocytes in blood was significant(P0.05)or highly significant(P0.01)differences between 50,100 μg groups and 200 μg group,controls after vaccination.100 μg group was the best one and the immune response of the 200 μg group was worse than pcDNA3.1(+).②100 μg group induced best cellular immune response of CD4+T cells,50 μg group was the better one.③50 μg group induced best cellular immune response of CD8+T cells,100 μg group was the better one.