Activator protein-2 inhibits survivin gene expression in Hela cells

Objective To elucidate the impacts of activator protein-2 (AP-2) on survivin gene expression in Hela cells and to provide a theoretical and clinical basis for targeted suppression of survivin gene. Methods The survivin promoter and AP-2 were cotransfected into Hela cells to detect the impacts of AP-2 on activities of survivin promoter. The regulation of the survivin expression by AP-2 was detected with RT-PCR and Western blotting after transfection. Results AP-2 was shown to inhibit the activity of survivin core promoter in Hela cells. In addition, AP-2 also inhibited survivin expression at the transcription and translation levels in Hela cells. Conclusion Inhibition of survivin expression in Hela cells by AP-2 may open up a new approach for effective regulation of survivin gene and provide a potential base for survivin-targeted cancer therapy.