Extraction of the volatile oil of Virgate Wormwood herb and preparation of β-cyclodextrin inclusion compound of Hugan granules

OBJECTIVE To optimize the condition of the process for mixture extraction of the volatile oil of Virgate Wormwood herb and other materials and the preparation of β-CYD inclusion compound of granules.METHODS Taking the weight of the volatile oil to evaluate the mixture extraction process and percentage recovery of inclusion compound,transfer rate of oil to evaluate the inclusion process,orthogonal design was applied with thin layer chromotography detecting the main content change of the volatile oil before and after inclusion.RESULTS The best volatile oil extraction condition was to add 6 folds water into the crud drugs,to soak for 1 hour and to reflux for 6 hours.And the optimum inclusion condition was β-CYD: volatile oil in proportion as 6∶1 was mixed in the saturated solution of β-CYD,refluxing and stirring for 1h at 50 ℃.Thin layer chromotographic discrimination showed that the main contents of the volatile oil before inclusion were the same as the ones after inclusion.CONCLUSION The best condition of the process for mixture extraction of volatile oil and preparation of β-CYD inclusion compound of Hugan granules are available for the development and production.