The comparison of five natural phenolic compounds on antioxidant activity in vitro

The antioxidant activity of caffeic acid,rutin,resveratrol,phloretin,and phlorizin were evaluated compared with VC,BHT and EDTA. Five areas were tested: the total antioxidant capacity,DPPH free radicals scavenging activity,ABTS free radicals scavenging activity,ferric reducing ability and ferrous ions chelating activity. The results indicated that caffeic acid showed strong total antioxidant capacity,DPPH radical scavenging activity( IC500. 023 ± 0. 004 mg /mL) and ferric reducing ability,the total antioxidant capacity reached 1. 873 ± 0. 022 mg VCeq /mg at a concentration of 0. 1 mg /mL; Resveratrol showed a strong ABTS radical scavenging activity( IC500. 056 ±0. 006 mg /mL),significantly higher than that of BHT and VC( P 0.01). Rutin showed the strongest ferrous ions chelating activity,followed by resveratrol. Other phenolic compounds showed little ferrous ions chelating activity. All the above tests showed 5 compounds have the potential natural antioxidants.