Detection of Enterotoxigenic Escherichia coli in food by LAMP

Enterotoxigenic Escherichia Coli (ETEC) is an important pathogen of zoonose, which can cause watery stools and acidosis. Thus, the development of a technique that can identify ETEC quickly and accurately is of great importance. LAMP primers were designed according to highly conserved heat-labile enterotoxin LT gene of Enterotoxigenic Escherichia Coli. The results show that the detection sensitivity of the LAMP method for Enterotoxigenic Escherichia Coli purely cultured was 32.9 cfu/mL and for Enterotoxigenic Escherichia Coli in contaminated meat was 55 cfu/g. LAMP has the potential to replace PCR, which can build a technology platform for the rapid detection of food-borne pathogens.