2007•Haiyang kexueRequires access
Preparation of spheroplasts from Spirulina platensis by ultrasonic-lysozyme method
Zhigang Wang
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Abstract
The obtainment of spheroplasts of Spirulina platensis by the improved ultrasonic-lysozyme method was dealt with in the paper.S.platensis was broken by ultrasonic,and the gelatinous sheath of the cells was rinsed with Zarrouk medium containing 1.5 mol/L NaCl.Then the cells were digested by lysozyme at 28~30℃ for 5~7 hours,the enzyme solution was composed of 0.8 mol/L KCl,0.5% lysozyme and 7.2 pH,0.2 mol/L buffer of K_2HPO_4 and KH_2PO_4.Results showed that spheroplasts rate could be obtained above 40%.