Comparative Study on Quality of Dendrobium officinale from Different Habits

To screen the elite germplasm of Dendrobium officinale,the content of polysaccharides,D-mannose and erianin from different habits were studied in this paper.According to the methods from the Pharmacopeia of the People's Repubic of China,the content of polysaccharides was determined by phenol-vitriol colorimetric,and using Pre-column derivatization-HPLC measured D-mannose content,and the content of erianin determined by HPLC.The results showed that a good correlation(r = 0.9987) between glucose level and Absorbance values at range from 0.018 to 0.090 mg.The content of polysaccharides of Dendrobium officinale ranged from 25.15% to 36.53%.Pre-column derivatization-HPLC method for the determination of D-mannose content was specific,accurate,reproducible.The D-mannose content of Dendrobium officinale ranged from 18.00% to 32.25%.The contents of erianin ranged from 0 to 0.0043%.There were singnificantly difference in the content of polysaccharides,D-mannose and erianin of Dendrobium officinale from different habits.