Detection of the faeces of animals inoculated with E.coli O157:H7

Objective To investigate the application of the gold immunochromatographic strip for the detection of animals inoculated with E.coli O157:H7.Methods The cattle and mice were inoculated intragastrically with E.coli O157:H7,and their faeces were detected by using the gold immunochromatographic strip and counting colony.Results E.coli O157:H7 in faeces could be detected on day 2 after inoculation in inoculated cattle by using immunochromatographic strip of E.coli O157:H7 and the method of plate culture count with differential medium of E.coli O157:H7 and the peak levels of O157:H7 in faeces were attained during the forth to sixth day after inoculation and then it dropped down gradually up to day 28 following inoculation.The O157:H7 in faeces could be detected on hour 4 after inoculation in inoculated mice by using IC strip of E.coli O157:H7 and the method of plate culture count with differential medium of E.coli O157:H7 and the peak levels of O157:H7 in faeces were attained during the sixth hour after inoculation and then it dropped down gradually up to day 15 following inoculation.Conclusion The gold immunochromatographic strip for the detection of E.coli O157:H7 is convenient and rapid for the clinical diagnosis and field investigation of E.coli O157:H7 infection or/and contamination.