Effects of Bio-compound Fertilizer on Corn Soil Microbial Community and Enzyme Activities

Fertilization could influence soil physiochemical and biological properties.By the biolog micro-plate technique,this study investigated the effects of bio-compound fertilizer,organic-inorganic fertilizer,inorganic fertilizer at the same nutrient amount of N,P and K on functional diversities of soil microbe communities and soil enzyme activities under corn potted experiment in greenhouse.The substrates of Biolog ECO plate were composed of six types of substances(carbohydrates,carboxylic acids,amino acids,polymers,amines and aromatic chemicals).Principal component analysis was used to show the difference utilization of substrates by soil microbes.The results showed that the Average Well-Color Developments(AWCD),Shannon index(H),Richness index(S)and Shannon evenness index(E)of microbe communities appeared the highest in bio-compound fertilizer treatment.The principal component analysis(PCA)showed that the carbon source use patterns of the soil microbe communities differed to a certain extent under different fertilization treatments,and the bio-compound fertilizer treatment showed positive values on PC1,and the other treatments showed negative values on PC1,and the major carbon sources to determine their differences were carbohydrates,carboxylic acids and amino acids.The soil invertase and urease activities also appeared the highest in bio-compound fertilizer treatment,reaching 72.74 mg glucose·g-1·(24 h)-1 and 1.15 mg NH3-N·g-1·(3 h)-1,respectively.At the same nutrient rates,application of the bio-compound fertilizer could significantly improve the functional diversities of soil microbe communities and increase the soil enzyme activities.