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CAFFEINT CAUSED THE RELEASE OF CALCITONIN GENE-RELATED PEPTIDE FROM THE SLICES OF RAT SPINAL CORED IN VITRO

Tang Yuemh

Abstract

Objective:Effects of caffeine on the release of calcitonin gene-related peptide(CGRP)from rat spinal cord in vitro and its mechanism were determined.Methods:Spinal cord slices in rats and ra-dioimmunoassay of CGRP,Results:Caffeine(1～10 mmol/L)caused concentration-dependent release of CGRP from rat spinal cord in vitro. In the absence of calcium in the Krebs solution the effect of caf-feine was not changed at 0～10 min,but decreased at 10～20 min. Caffeine induced-CGRP release was inhibited by pretreatment with ruthenium red or ryanodine,respectively.Conclusion:The data sug-gested that caffeine directly evokes CGRP release by triggering calcium release from ryanodine-sensitive intracellular calcium store in rat spinal cord.

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Key concepts: Calcitonin gene-related peptide, Caffeine, Spinal cord, Ryanodine receptor, Chemistry, Ruthenium red, In vitro, Calcium