Analysis of correlation of quantitative fluorescent detection of HBV DNA and the results of serum marker

Objective To analyze the relationship between the level of HBV DNA and the HBV-M. Methods Serum samples from 1 781 service workers were detected by ELISA and divided into nine groups: HBsAg(+)/HBeAg(+)/HBcAb(+),HBsAg(+)/HBeAb(+)/HBcAb(+),HBsAg(+)/HBcAb(+),HBsAg(+)/HBeAg(+),HBsAb/HBcAb(+),HBeAb(+)/HBcAb(+),HBsAg(+)/HBeAb(+),HBsAb(+) and HBsAg(+).HBV DNA concentration was determined by fluorescent quantitative PCR. Results The positive rate of HBV-DNA in the group of HBsAg(+)/HBeAg(+)/HBcAb(+) was 61.88%(1 102/1 781),the positive rate in the group of HBsAg(+)/HBeAb(+)/HBcAb(+) was 96.15%,the positive rate in the group of HBsAg(+)/HBeAb(+)/HBcAb(+) was 24.38%,the positive rate in the group of HBsAg(+)/HBcAb(+) was 38.41%,90.91% in the group of HBsAg(+)/HBeAg(+),36.36% in the group of HBeAg(+) and the positive rate of HBV-DNA was 0% in the other groups.There significant differences between the different hepatitis B sera positive modes and the result of HBV DNA.The HBV DNA level showed an obvious correlation with the HBeAg content in sera. Conclusion A close correlation exists between these two methods in the detection of HBV markers.The FQ-PCR is a relatively acurate method for detection of HBV-DNA.