2008•Journal of Clinical Medicine in PracticeRequires access
Effect of antiangiogenesis of thalidomide and methotrexate on rats with collagen type II induced arthritis
Li Ma
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Abstract
Objective To investigate the effect of thalidomide (THD) and methotrexate (MTX) on rats with collagen typeⅡinduced arthritis,and explore the mechanism of inhibiting an- giogenesis on model rats.Methods The model of rat was induced by injection of collagen typeⅡcombined with complete Freund's adjuvant (CFA).Next day after the injection,the therapeutic administration of thalidomide,methotrexate and thalidomide combined with methotrexate were dif- ferently performed to the rats.Knee joints from the rats sacrificed at the sixth week were estimated in morphology,and the microvessel density (MVD) of synovial membrane was detected using im- munohistochemistry assay.The relative concentration of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-1,2,3,9 in sera were evaluated by Western Blotting.Results①The therapeutic administration of THD and MTX or THD+MTX can inhibite antiangiogenesis and decrease the microvessel density (MVD) of synovial membrane on CIA rat.There were more obvi- rous effect in THD+MTX group.②The administration of THD and MTX or THD+MTX signifi- cantly decreased the expression of VEGF、MMP-1,2,3,9 in sera of CIA rat.Conclusions THD and MTX had an inhibitory effect on the expression of VEGF、MMP-1,2,3,9 in model rat, which was related to its antiangiogenesis.There were the Synergetic effect on THD and MTX.