Clinical significance and culture of hematopoietic progenitor cells in aplastic anemia

Objective To study hematopoietic progenitor cells(HPC) proliferation through detection of blast-forming-unit of erythyrocyte(BFU-E),colony forming-unit of erythrocyte(CFU-E),colony-forming-unit of granulocyte-monocyte(CFU-GM) and colony-forming-unit of megakayocyte(CFU-Meg) by HPC culture of bone marrow(BM) from aplastic anemia(AA).Methods Detecting BFU-E,CFU-E,CFU-GM and CFU-Meg at 7 d,14 d after cellular culture from 39 cases with AA.Results The mean values of four different colonies in 39 patients were significantly lower than those of the control.The mean decrease of these colonies was positively correlated with the severity of the types of diseases.Conclusion A portion of cases with AA have lower quantities of BFU-E,CFU-E,CFU-GM and CFU-Meg,and the quantitative decrease in these colonies is positively correlated with severity of the disease.Testing the changes of BFU-E,CFU-E,CFU-GM and CFU-Meg by HPC culture has significance to the diagnosis,effect observeing and prognosis judging.