Effect of p115 gene silence on migration and invasion abilities of human gastric cancer BGC-823 cells and relevant mechanism

Objective To investigate the effect of Golgi-vesicular transport protein p115 gene silence on migration and invasion abilities of human gastric cancer BGC-823 cells as well as the relevant mechanism.Methods BGC-823 cells transfected with plasmid pGPU6/GFP/Neo/p115(p115 shRNA) and negative control plasmid(shNC) in mediation of liposome respectively,using those untransfected as blank control.Forty-eight hours after transfection,the expressions of p115,macrophage migration inhibitory factor(MIF),matrix metalloproteinase-2(MMP-2) and MMP-9 genes and proteins in various groups were determined by RT-PCR,Western blot and IFA,while the invasion ability of cells by wound healing test and Transwell test.Results Compared with those in shNC and blank control groups,the expression levels of p115,MIF,MMP-2 and MMP-9 gene and protein as well as the healing ability and the number of transmembrane cells in p115 shRNA group decreased significantly(each P 0.01).Conclusion Inhibition of P115 expression in BGC-823 cells inhibited the invasion ability of tumor cells by down-regulating the expressions of MIF,MMP-2 and MMP-9 genes.