Effects of ox-LDL on the Proaggregation and Proadhesion-related Molecules Expression of Vascular Endothelial Cells

Aim The effect of oxidized low density lipoprotein( ox-LDL) on proaggregation and proadhesion in human umbilical vein endothelial cells( HUVEC) and the underlying mechanisms were observed. Methods HUVEC in 6-well plate were treated with varied concentrations of ox-LDL( 50,100 and 200 mg / L) for 12 h,24 h and 48 h,respectively. After that,the levels of prostacyclin( PGI2) and thromboxane A2( TXA2) in supernatants were detected by radioimmunoasssay. Finally,the expression levels of tissue factor( TF),intercellular cell adhesion molecule-1( ICAM-1) and vascular cell adhesion molecule-1( VCAM-1) were examined by Western blot. Results Dose-response assay showed that the proaggregation of HUVEC induced by ox-LDL was enhanced in a concentration-dependent manner by treatment with ox-LDL for 24 h,presenting that the ratios of PGI2 / TXA2 markedly decreased( P 0. 05 or P 0. 01). The similar results from time-course relationship assay were obtained. In addition,the expression levels of TF significantly increased in a fashion of concentration-dependence. More importantly,the proadhesion of HUVEC was enhanced,displaying the expression levels of ICAM-1 and VCAM-1 were significantly increased in a concentration-dependent fashion. Conclusions ox-LDL enhances the activities of proaggregation and proadhesion in HUVEC in a concentration-dependent manner. The underlying mechanisms might downregulate the value of PGI2 / TXA2 and upregulate the expression of TF, ICAM-1 and VCAM-1.